![]() Illumina Infinium HumanMethylation450 Beadchip arrays, whole-genome bisulfite sequencing) have driven research in this area over the past decade, and a key feature of many DNA methylation assays is the use of the bisulfite treatment process. Advancements in genome-wide methylation analysis technologies (e.g. This resource is freely available for use at PrimerSuite website ( The methylation of cytosine at the carbon-5 position (5-methylcytosine) is an epigenetic mark associated with the regulation of numerous cellular processes in the mammalian genome such as embryonic development, genomic imprinting, X chromosome inactivation, and preservation stability 1, 2, and aberrant patterns of DNA methylation have been implicated in various pathologies such as cancer. The potential use of the software in other bisulfite-based applications such as methylation-specific PCR is under consideration for future updates. Moreover, a major focus in the development of this software package was the emphasis on extensive empirical validation, and over 1300 unique primer pairs have been successfully designed and screened, with over 94% of them producing amplicons of the expected size, and an average mapping efficiency of 93% when screened using bisulfite multiplex resequencing. This software was constructed to (i) design bisulfite primers against multiple regions simultaneously ( PrimerSuite ), (ii) screen for primer-primer dimerizing artefacts ( PrimerDimer ), and (iii) support multiplex PCR assays ( PrimerPlex ). ![]() ![]() In response, the tri-modular software package PrimerSuite was developed to support bisulfite multiplex PCR applications. ![]() Moreover, a review of the literature indicated no available software solutions which accommodated both high-throughput primer design, support for multiplex amplification assays, and primer-dimer prediction. The analysis of DNA methylation at CpG dinucleotides has become a major research focus due to its regulatory role in numerous biological processes, but the requisite need for assays which amplify bisulfite-converted DNA represents a major bottleneck due to the unique design constraints imposed on bisulfite-PCR primers. ![]()
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |